Putative endoglucanase PcGH5 from Phanerochaete chrysosporium is a β-xylosidase that cleaves xylans in synergistic action with endo-xylanase.
Identifieur interne : 000253 ( Main/Exploration ); précédent : 000252; suivant : 000254Putative endoglucanase PcGH5 from Phanerochaete chrysosporium is a β-xylosidase that cleaves xylans in synergistic action with endo-xylanase.
Auteurs : Nguyen Duc Huy [Corée du Sud] ; Cu Le Nguyen [Corée du Sud] ; Jeong-Woo Seo [Corée du Sud] ; Dae-Hyuk Kim [Corée du Sud] ; Seung-Moon Park [Corée du Sud]Source :
- Journal of bioscience and bioengineering [ 1347-4421 ] ; 2015.
Descripteurs français
- KwdFr :
- Biocatalyse (MeSH), Cellulase (composition chimique), Cellulase (métabolisme), Clonage moléculaire (MeSH), Concentration en ions d'hydrogène (MeSH), Endo-1,4-beta xylanases (composition chimique), Endo-1,4-beta xylanases (génétique), Endo-1,4-beta xylanases (isolement et purification), Endo-1,4-beta xylanases (métabolisme), Glycosylation (MeSH), Hordeum (composition chimique), Hydrolyse (MeSH), Phanerochaete (enzymologie), Phanerochaete (génétique), Pichia (métabolisme), Polyosides (métabolisme), Spécificité du substrat (MeSH), Structure tertiaire des protéines (MeSH), Température (MeSH), Xylanes (métabolisme), Xylose (métabolisme), Xylosidases (composition chimique), Xylosidases (génétique), Xylosidases (isolement et purification), Xylosidases (métabolisme), beta-Galactosidase (composition chimique), beta-Galactosidase (métabolisme).
- MESH :
- composition chimique : Cellulase, Endo-1,4-beta xylanases, Hordeum, Xylosidases, beta-Galactosidase.
- enzymologie : Phanerochaete.
- génétique : Endo-1,4-beta xylanases, Phanerochaete, Xylosidases.
- isolement et purification : Endo-1,4-beta xylanases, Xylosidases.
- métabolisme : Cellulase, Endo-1,4-beta xylanases, Pichia, Polyosides, Xylanes, Xylose, Xylosidases, beta-Galactosidase.
- Biocatalyse, Clonage moléculaire, Concentration en ions d'hydrogène, Glycosylation, Hydrolyse, Spécificité du substrat, Structure tertiaire des protéines, Température.
English descriptors
- KwdEn :
- Biocatalysis (MeSH), Cellulase (chemistry), Cellulase (metabolism), Cloning, Molecular (MeSH), Endo-1,4-beta Xylanases (chemistry), Endo-1,4-beta Xylanases (genetics), Endo-1,4-beta Xylanases (isolation & purification), Endo-1,4-beta Xylanases (metabolism), Glycosylation (MeSH), Hordeum (chemistry), Hydrogen-Ion Concentration (MeSH), Hydrolysis (MeSH), Phanerochaete (enzymology), Phanerochaete (genetics), Pichia (metabolism), Polysaccharides (metabolism), Protein Structure, Tertiary (MeSH), Substrate Specificity (MeSH), Temperature (MeSH), Xylans (metabolism), Xylose (metabolism), Xylosidases (chemistry), Xylosidases (genetics), Xylosidases (isolation & purification), Xylosidases (metabolism), beta-Galactosidase (chemistry), beta-Galactosidase (metabolism).
- MESH :
- chemical , chemistry : Cellulase, Endo-1,4-beta Xylanases, Xylosidases, beta-Galactosidase.
- chemical , genetics : Endo-1,4-beta Xylanases, Xylosidases.
- chemical , isolation & purification : Endo-1,4-beta Xylanases, Xylosidases.
- chemical , metabolism : Cellulase, Endo-1,4-beta Xylanases, Polysaccharides, Xylans, Xylose, Xylosidases, beta-Galactosidase.
- chemistry : Hordeum.
- enzymology : Phanerochaete.
- genetics : Phanerochaete.
- metabolism : Pichia.
- Biocatalysis, Cloning, Molecular, Glycosylation, Hydrogen-Ion Concentration, Hydrolysis, Protein Structure, Tertiary, Substrate Specificity, Temperature.
Abstract
A predicted endoglucanase gene (PcGH5) was cloned from Phanerochaete chysosporium, and expressed in Pichia pastoris. Although PcGH5 showed similarity with the conserved domains of a cellulase superfamily GH5, a β-glucosidase/6-phospho-β-glucosidase/β-galactosidase superfamily, and an endoglucanase, recombinant PcGH5 exhibited a β-xylosidase activity, rather than endoglucanase activity. Therefore, the predicted gene was named as PcXyl5. Further characterization of recombinant PcXyl5 showed not only catalysis of the hydrolysis of xylo-oligomers to xylose, but also displayed transglycosylation activity using alcohol as a receptor. Optimum pH of rPcXyl5 was found to be 5.5, whereas optimum temperature was 50°C. rPcXyl5 increased reducing sugar release of birchwood xylan, beechwood xylan, and arabinoxylan by 6.4%, 13%, 15.8%, respectively, in synergistic action with endo-xylanase. Interestingly, the late addition of rPcXyl5 into reaction with endo-xylanase resulted in a larger increase of reducing sugar release from pretreated barley straw that addition at the start or by treatment with endo-xylanases alone. The increases observed were 6.3% and 13.8%, respectively, showing a great potential application for hemicellulose saccharification.
DOI: 10.1016/j.jbiosc.2014.09.012
PubMed: 25300189
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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Electronic address: smpark@chonbuk.ac.kr.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Division of Biotechnology, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan, Jeonbuk 570-752</wicri:regionArea><wicri:noRegion>Jeonbuk 570-752</wicri:noRegion></affiliation></author></analytic><series><title level="j">Journal of bioscience and bioengineering</title><idno type="eISSN">1347-4421</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Biocatalysis (MeSH)</term><term>Cellulase (chemistry)</term><term>Cellulase (metabolism)</term><term>Cloning, Molecular (MeSH)</term><term>Endo-1,4-beta Xylanases (chemistry)</term><term>Endo-1,4-beta Xylanases (genetics)</term><term>Endo-1,4-beta Xylanases (isolation & purification)</term><term>Endo-1,4-beta Xylanases (metabolism)</term><term>Glycosylation (MeSH)</term><term>Hordeum (chemistry)</term><term>Hydrogen-Ion Concentration (MeSH)</term><term>Hydrolysis (MeSH)</term><term>Phanerochaete (enzymology)</term><term>Phanerochaete (genetics)</term><term>Pichia (metabolism)</term><term>Polysaccharides (metabolism)</term><term>Protein Structure, Tertiary (MeSH)</term><term>Substrate Specificity (MeSH)</term><term>Temperature (MeSH)</term><term>Xylans (metabolism)</term><term>Xylose (metabolism)</term><term>Xylosidases (chemistry)</term><term>Xylosidases (genetics)</term><term>Xylosidases (isolation & purification)</term><term>Xylosidases (metabolism)</term><term>beta-Galactosidase (chemistry)</term><term>beta-Galactosidase (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Biocatalyse (MeSH)</term><term>Cellulase (composition chimique)</term><term>Cellulase (métabolisme)</term><term>Clonage moléculaire (MeSH)</term><term>Concentration en ions d'hydrogène (MeSH)</term><term>Endo-1,4-beta xylanases (composition chimique)</term><term>Endo-1,4-beta xylanases (génétique)</term><term>Endo-1,4-beta xylanases (isolement et purification)</term><term>Endo-1,4-beta xylanases (métabolisme)</term><term>Glycosylation (MeSH)</term><term>Hordeum (composition chimique)</term><term>Hydrolyse (MeSH)</term><term>Phanerochaete (enzymologie)</term><term>Phanerochaete (génétique)</term><term>Pichia (métabolisme)</term><term>Polyosides (métabolisme)</term><term>Spécificité du substrat (MeSH)</term><term>Structure tertiaire des protéines (MeSH)</term><term>Température (MeSH)</term><term>Xylanes (métabolisme)</term><term>Xylose (métabolisme)</term><term>Xylosidases (composition chimique)</term><term>Xylosidases (génétique)</term><term>Xylosidases (isolement et purification)</term><term>Xylosidases (métabolisme)</term><term>beta-Galactosidase (composition chimique)</term><term>beta-Galactosidase (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Cellulase</term><term>Endo-1,4-beta Xylanases</term><term>Xylosidases</term><term>beta-Galactosidase</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Endo-1,4-beta Xylanases</term><term>Xylosidases</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Endo-1,4-beta Xylanases</term><term>Xylosidases</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Cellulase</term><term>Endo-1,4-beta Xylanases</term><term>Polysaccharides</term><term>Xylans</term><term>Xylose</term><term>Xylosidases</term><term>beta-Galactosidase</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Hordeum</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Cellulase</term><term>Endo-1,4-beta xylanases</term><term>Hordeum</term><term>Xylosidases</term><term>beta-Galactosidase</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Phanerochaete</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Phanerochaete</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Phanerochaete</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Endo-1,4-beta xylanases</term><term>Phanerochaete</term><term>Xylosidases</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Endo-1,4-beta xylanases</term><term>Xylosidases</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Pichia</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Cellulase</term><term>Endo-1,4-beta xylanases</term><term>Pichia</term><term>Polyosides</term><term>Xylanes</term><term>Xylose</term><term>Xylosidases</term><term>beta-Galactosidase</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Biocatalysis</term><term>Cloning, Molecular</term><term>Glycosylation</term><term>Hydrogen-Ion Concentration</term><term>Hydrolysis</term><term>Protein Structure, Tertiary</term><term>Substrate Specificity</term><term>Temperature</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Biocatalyse</term><term>Clonage moléculaire</term><term>Concentration en ions d'hydrogène</term><term>Glycosylation</term><term>Hydrolyse</term><term>Spécificité du substrat</term><term>Structure tertiaire des protéines</term><term>Température</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A predicted endoglucanase gene (PcGH5) was cloned from Phanerochaete chysosporium, and expressed in Pichia pastoris. Although PcGH5 showed similarity with the conserved domains of a cellulase superfamily GH5, a β-glucosidase/6-phospho-β-glucosidase/β-galactosidase superfamily, and an endoglucanase, recombinant PcGH5 exhibited a β-xylosidase activity, rather than endoglucanase activity. Therefore, the predicted gene was named as PcXyl5. Further characterization of recombinant PcXyl5 showed not only catalysis of the hydrolysis of xylo-oligomers to xylose, but also displayed transglycosylation activity using alcohol as a receptor. Optimum pH of rPcXyl5 was found to be 5.5, whereas optimum temperature was 50°C. rPcXyl5 increased reducing sugar release of birchwood xylan, beechwood xylan, and arabinoxylan by 6.4%, 13%, 15.8%, respectively, in synergistic action with endo-xylanase. Interestingly, the late addition of rPcXyl5 into reaction with endo-xylanase resulted in a larger increase of reducing sugar release from pretreated barley straw that addition at the start or by treatment with endo-xylanases alone. The increases observed were 6.3% and 13.8%, respectively, showing a great potential application for hemicellulose saccharification. </div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" IndexingMethod="Curated" Owner="NLM"><PMID Version="1">25300189</PMID><DateCompleted><Year>2015</Year><Month>07</Month><Day>06</Day></DateCompleted><DateRevised><Year>2018</Year><Month>12</Month><Day>02</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1347-4421</ISSN><JournalIssue CitedMedium="Internet"><Volume>119</Volume><Issue>4</Issue><PubDate><Year>2015</Year><Month>Apr</Month></PubDate></JournalIssue><Title>Journal of bioscience and bioengineering</Title><ISOAbbreviation>J Biosci Bioeng</ISOAbbreviation></Journal><ArticleTitle>Putative endoglucanase PcGH5 from Phanerochaete chrysosporium is a β-xylosidase that cleaves xylans in synergistic action with endo-xylanase.</ArticleTitle><Pagination><MedlinePgn>416-20</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.jbiosc.2014.09.012</ELocationID><ELocationID EIdType="pii" ValidYN="Y">S1389-1723(14)00347-8</ELocationID><Abstract><AbstractText>A predicted endoglucanase gene (PcGH5) was cloned from Phanerochaete chysosporium, and expressed in Pichia pastoris. Although PcGH5 showed similarity with the conserved domains of a cellulase superfamily GH5, a β-glucosidase/6-phospho-β-glucosidase/β-galactosidase superfamily, and an endoglucanase, recombinant PcGH5 exhibited a β-xylosidase activity, rather than endoglucanase activity. Therefore, the predicted gene was named as PcXyl5. Further characterization of recombinant PcXyl5 showed not only catalysis of the hydrolysis of xylo-oligomers to xylose, but also displayed transglycosylation activity using alcohol as a receptor. Optimum pH of rPcXyl5 was found to be 5.5, whereas optimum temperature was 50°C. rPcXyl5 increased reducing sugar release of birchwood xylan, beechwood xylan, and arabinoxylan by 6.4%, 13%, 15.8%, respectively, in synergistic action with endo-xylanase. Interestingly, the late addition of rPcXyl5 into reaction with endo-xylanase resulted in a larger increase of reducing sugar release from pretreated barley straw that addition at the start or by treatment with endo-xylanases alone. The increases observed were 6.3% and 13.8%, respectively, showing a great potential application for hemicellulose saccharification. </AbstractText><CopyrightInformation>Copyright © 2014 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Huy</LastName><ForeName>Nguyen Duc</ForeName><Initials>ND</Initials><AffiliationInfo><Affiliation>Division of Biotechnology, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan, Jeonbuk 570-752, Republic of Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Nguyen</LastName><ForeName>Cu Le</ForeName><Initials>CL</Initials><AffiliationInfo><Affiliation>Division of Biotechnology, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan, Jeonbuk 570-752, Republic of Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Seo</LastName><ForeName>Jeong-Woo</ForeName><Initials>JW</Initials><AffiliationInfo><Affiliation>Applied Microbiology Research Center, Bio-Materials Research Institute, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Jeongeup, Jeonbuk 580-185, Republic of Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kim</LastName><ForeName>Dae-Hyuk</ForeName><Initials>DH</Initials><AffiliationInfo><Affiliation>Department of Molecular Biology, College of Natural Sciences, Chonbuk National University, Jeonju, Jeonbuk 561-756, Republic of Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Park</LastName><ForeName>Seung-Moon</ForeName><Initials>SM</Initials><AffiliationInfo><Affiliation>Division of Biotechnology, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan, Jeonbuk 570-752, Republic of Korea. Electronic address: smpark@chonbuk.ac.kr.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2014</Year><Month>10</Month><Day>07</Day></ArticleDate></Article><MedlineJournalInfo><Country>Japan</Country><MedlineTA>J Biosci Bioeng</MedlineTA><NlmUniqueID>100888800</NlmUniqueID><ISSNLinking>1347-4421</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011134">Polysaccharides</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D014990">Xylans</NameOfSubstance></Chemical><Chemical><RegistryNumber>8024-50-8</RegistryNumber><NameOfSubstance UI="C007916">hemicellulose</NameOfSubstance></Chemical><Chemical><RegistryNumber>9040-27-1</RegistryNumber><NameOfSubstance UI="C085118">arabinoxylan</NameOfSubstance></Chemical><Chemical><RegistryNumber>A1TA934AKO</RegistryNumber><NameOfSubstance UI="D014994">Xylose</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.2.1.-</RegistryNumber><NameOfSubstance UI="D014995">Xylosidases</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.2.1.23</RegistryNumber><NameOfSubstance UI="D001616">beta-Galactosidase</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.2.1.37</RegistryNumber><NameOfSubstance UI="C026579">exo-1,4-beta-D-xylosidase</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.2.1.4</RegistryNumber><NameOfSubstance UI="D002480">Cellulase</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.2.1.8</RegistryNumber><NameOfSubstance UI="D043364">Endo-1,4-beta Xylanases</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D055162" MajorTopicYN="N">Biocatalysis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002480" MajorTopicYN="N">Cellulase</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D003001" MajorTopicYN="N">Cloning, Molecular</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D043364" MajorTopicYN="N">Endo-1,4-beta Xylanases</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006031" MajorTopicYN="N">Glycosylation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001467" MajorTopicYN="N">Hordeum</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006863" MajorTopicYN="N">Hydrogen-Ion Concentration</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006868" MajorTopicYN="N">Hydrolysis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D020075" MajorTopicYN="N">Phanerochaete</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="Y">enzymology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010843" MajorTopicYN="N">Pichia</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011134" MajorTopicYN="N">Polysaccharides</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017434" MajorTopicYN="N">Protein Structure, Tertiary</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013379" MajorTopicYN="N">Substrate Specificity</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013696" MajorTopicYN="N">Temperature</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014990" MajorTopicYN="N">Xylans</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014994" MajorTopicYN="N">Xylose</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014995" MajorTopicYN="N">Xylosidases</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001616" MajorTopicYN="N">beta-Galactosidase</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Glycoside hydrolase family 5</Keyword><Keyword MajorTopicYN="N">Hemicellulose</Keyword><Keyword MajorTopicYN="N">Phanerochaete chrysosporium</Keyword><Keyword MajorTopicYN="N">Xylan</Keyword><Keyword MajorTopicYN="N">Xylosidase</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2014</Year><Month>05</Month><Day>27</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2014</Year><Month>08</Month><Day>12</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2014</Year><Month>09</Month><Day>14</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2014</Year><Month>10</Month><Day>11</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2014</Year><Month>10</Month><Day>11</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2015</Year><Month>7</Month><Day>7</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">25300189</ArticleId><ArticleId IdType="pii">S1389-1723(14)00347-8</ArticleId><ArticleId IdType="doi">10.1016/j.jbiosc.2014.09.012</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Corée du Sud</li></country></list><tree><country name="Corée du Sud"><noRegion><name sortKey="Huy, Nguyen Duc" sort="Huy, Nguyen Duc" uniqKey="Huy N" first="Nguyen Duc" last="Huy">Nguyen Duc Huy</name></noRegion><name sortKey="Kim, Dae Hyuk" sort="Kim, Dae Hyuk" uniqKey="Kim D" first="Dae-Hyuk" last="Kim">Dae-Hyuk Kim</name><name sortKey="Nguyen, Cu Le" sort="Nguyen, Cu Le" uniqKey="Nguyen C" first="Cu Le" last="Nguyen">Cu Le Nguyen</name><name sortKey="Park, Seung Moon" sort="Park, Seung Moon" uniqKey="Park S" first="Seung-Moon" last="Park">Seung-Moon Park</name><name sortKey="Seo, Jeong Woo" sort="Seo, Jeong Woo" uniqKey="Seo J" first="Jeong-Woo" last="Seo">Jeong-Woo Seo</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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|wiki= Bois
|area= PhanerochaeteV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:25300189
|texte= Putative endoglucanase PcGH5 from Phanerochaete chrysosporium is a β-xylosidase that cleaves xylans in synergistic action with endo-xylanase.
}}
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